W130i Specifications

Measurement Principle Dynamic Light Scattering PDFimg
Download W130i Brochure PDF
Measurement Range (hydrodynamic diameter) 0.3 to 1000nm
Measurement Time (typical) 30 sec
Sample Liquid Volume (min – max) 2 – 4500μL & online
Light Source 660nm; 30mW
Detector Type Avalanche Photo Diode
Temperature Control Range 0°C – 90°C
Temperature Control Accuracy ±0.2°C
Cuvette Type quartz or disposable
Computer Communication 1 x USB
Environmental Temperature: 10°C to 40°C
Humidity: 10% to 90% (without condensation)
Power Supply AC 100V ~ 240V, 50/60 Hz universal input
Size and Weight 130 (W) x 340 (D) x 210 (H), 6.5kg
Computer OS Microsoft Windows XP / 7 (32 or 64 bit)
Validation Standards ISO13321 : 1996
ISO22412 : 2008
Accuracy ±2%
Polydispersity Index <0.1 (certified standard)
Min sample concentration 0.1g/L of lysozyme protein in phosphate buffer (2nm radius)
(much lower sample concentration required for larger particles)
Molecular Weight range 192 Da (e.g. caffeine) ~ 25MDa
Min sample volume 2μL (partially filled 12μL quartz cuvette)
Measurement temperature range 0 – 90 deg C
Sample cuvette types (typical) 5μL polycarbonate ‘BladeCell’ disposable (Avid Nano design)
Generic 12.5mm square walled cuvettes with 4 clear windows
8μL flow through cell
12μL quartz
45μL quartz
50μL polystyrene disposable (Eppendorf Uvette)
Measurements provided Mean hydrodynamic size (nm) (according to ISO13321)
Polydispersity Index (according to ISO13321)
Standard deviation (nm)
Mean photon count rate
Intensity Size Distribution
Mass Size Distribution
Peak distribution, SD, %mass analysis
Molecular Weight estimate (for biological molecules)
Molecular Weight measurement by batch static light scattering analysis
Online ‘Flow Mode’ chromatography analysis coming in 2012 (ask for details)