| Measurement Principle |
Dynamic Light Scattering |
| Measurement Range (hydrodynamic diameter) |
0.3 to 1000nm |
| Measurement Time (typical) |
30 sec |
| Sample Liquid Volume (min – max) |
2 – 4500μL & online |
| Light Source |
660nm; 30mW |
| Detector Type |
Avalanche Photo Diode |
| Temperature Control Range |
0°C – 90°C |
| Temperature Control Accuracy |
±0.2°C |
| Cuvette Type |
quartz or disposable |
| Computer Communication |
1 x USB |
| Environmental |
Temperature: 10°C to 40°C |
|
Humidity: 10% to 90% (without condensation) |
| Power Supply |
AC 100V ~ 240V, 50/60 Hz universal input |
| Size and Weight |
130 (W) x 340 (D) x 210 (H), 6.5kg |
| Computer OS |
Microsoft Windows XP / 7 (32 or 64 bit) |
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| Validation Standards |
ISO13321 : 1996 |
|
ISO22412 : 2008 |
| Accuracy |
±2% |
| Polydispersity Index |
<0.1 (certified standard) |
| Min sample concentration |
0.1g/L of lysozyme protein in phosphate buffer (2nm radius) |
|
(much lower sample concentration required for larger particles) |
| Molecular Weight range |
192 Da (e.g. caffeine) ~ 25MDa |
| Min sample volume |
2μL (partially filled 12μL quartz cuvette) |
| Measurement temperature range |
0 – 90 deg C |
| Sample cuvette types (typical) |
5μL polycarbonate ‘BladeCell’ disposable (Avid Nano design) |
|
Generic 12.5mm square walled cuvettes with 4 clear windows |
|
8μL flow through cell |
|
12μL quartz |
|
45μL quartz |
|
50μL polystyrene disposable (Eppendorf Uvette) |
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| Measurements provided |
Mean hydrodynamic size (nm) (according to ISO13321) |
|
Polydispersity Index (according to ISO13321) |
|
Standard deviation (nm) |
|
Mean photon count rate |
|
Intensity Size Distribution |
|
Mass Size Distribution |
|
Peak distribution, SD, %mass analysis |
|
Molecular Weight estimate (for biological molecules) |
|
Molecular Weight measurement by batch static light scattering analysis |
|
Online ‘Flow Mode’ chromatography analysis coming in 2012 (ask for details) |
